Analysis of the Diluents Used for Rubella Virus Hemagglutination and Hemagglutination-Inhibition Tests1
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چکیده
منابع مشابه
Analysis of the diluents used for rubella virus hemagglutination and hemagglutination-inhibition tests.
A new diluent (ADGP) for the rubella virus hemagglutination (HA) and hemagglutination-inhibition (HI) tests is described. It was found that the HA and HI titers and the erythrocyte agglutination pattern were improved in ADGP compared to previously described diluents. The influence of the components of ADGP and of various test conditions on optimal HA and HI results were examined.
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Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20x concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1969
ISSN: 0003-6919
DOI: 10.1128/aem.18.2.221-227.1969